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primary antibodies specific to perk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies specific to perk
    Primary Antibodies Specific To Perk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies specific to perk/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    primary antibodies specific to perk - by Bioz Stars, 2026-03
    90/100 stars

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    Effect of 10 days administration of nitazoxanide (NTZ-100 and NTZ-200) at 100 and 200 mg/kg, respectively, N-acetylcysteine (NAC) at 500 mg/kg and 4-Phenylbutyric acid (4-PBA) on hepatic endoplasmic reticulum (ER) stress. Hepatic ER-stress is expressed by hepatic levels of stress sensor protein, inositol-requiring enzyme 1 IRE1, (A) , and hepatic mRNA expression <t>of</t> <t>PERK,</t> (B) , GRP78, (C) , ATF4, (D) , and C/EBP homologous protein <t>CHOP-10,</t> (E) . Data are means ± SEM. * Vs. CTRL; # Vs. MTX group; ¥ Vs. NTZ-100 group, p < 0.05.
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    Effect of 10 days administration of nitazoxanide (NTZ-100 and NTZ-200) at 100 and 200 mg/kg, respectively, N-acetylcysteine (NAC) at 500 mg/kg and 4-Phenylbutyric acid (4-PBA) on hepatic endoplasmic reticulum (ER) stress. Hepatic ER-stress is expressed by hepatic levels of stress sensor protein, inositol-requiring enzyme 1 IRE1, (A) , and hepatic mRNA expression of PERK, (B) , GRP78, (C) , ATF4, (D) , and C/EBP homologous protein CHOP-10, (E) . Data are means ± SEM. * Vs. CTRL; # Vs. MTX group; ¥ Vs. NTZ-100 group, p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Nitazoxanide mitigates methotrexate hepatotoxicity in rats: role in inhibiting apoptosis and regulating endoplasmic reticulum stress

    doi: 10.3389/fphar.2024.1491249

    Figure Lengend Snippet: Effect of 10 days administration of nitazoxanide (NTZ-100 and NTZ-200) at 100 and 200 mg/kg, respectively, N-acetylcysteine (NAC) at 500 mg/kg and 4-Phenylbutyric acid (4-PBA) on hepatic endoplasmic reticulum (ER) stress. Hepatic ER-stress is expressed by hepatic levels of stress sensor protein, inositol-requiring enzyme 1 IRE1, (A) , and hepatic mRNA expression of PERK, (B) , GRP78, (C) , ATF4, (D) , and C/EBP homologous protein CHOP-10, (E) . Data are means ± SEM. * Vs. CTRL; # Vs. MTX group; ¥ Vs. NTZ-100 group, p < 0.05.

    Article Snippet: The membrane was blocked in tris-buffered saline with Tween 20 (TBST) and 3% bovine serum albumin (BSA) at room temperature for 1 h. The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4°C overnight, then the blots were rinsed 3–5 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature.

    Techniques: Expressing

    Effect of nitazoxanide on hepatic markers of endoplasmic reticulum stress. (A) Cropped Western blot gels for CHOP-10 and P-ERK proteins in the experimental groups (1) CNTR, (2) MTX, (3) MTX + NTZ-100, (4) NTZ-200 groups, MTX + NAC and MTX + 4-PBA. (B, C) Column charts for CHOP-10 and P-PERK levels. (D, E) Column charts for ATF4 and GRP78 levels. Data are means ± SEM. * Vs. CTRL; Vs. MTX group; ¥ Vs. NTZ-100 group, p < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Nitazoxanide mitigates methotrexate hepatotoxicity in rats: role in inhibiting apoptosis and regulating endoplasmic reticulum stress

    doi: 10.3389/fphar.2024.1491249

    Figure Lengend Snippet: Effect of nitazoxanide on hepatic markers of endoplasmic reticulum stress. (A) Cropped Western blot gels for CHOP-10 and P-ERK proteins in the experimental groups (1) CNTR, (2) MTX, (3) MTX + NTZ-100, (4) NTZ-200 groups, MTX + NAC and MTX + 4-PBA. (B, C) Column charts for CHOP-10 and P-PERK levels. (D, E) Column charts for ATF4 and GRP78 levels. Data are means ± SEM. * Vs. CTRL; Vs. MTX group; ¥ Vs. NTZ-100 group, p < 0.05.

    Article Snippet: The membrane was blocked in tris-buffered saline with Tween 20 (TBST) and 3% bovine serum albumin (BSA) at room temperature for 1 h. The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4°C overnight, then the blots were rinsed 3–5 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature.

    Techniques: Western Blot